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Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Complete Version

Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Complete Version - 10th edition

ISBN13: 978-0071254168

Cover of Benson
ISBN13: 978-0071254168
ISBN10: 0071254161
Cover type:
Edition/Copyright: 10TH 07
Publisher: McGraw-Hill Publishing Company
Published: 2007
International: No

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Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Complete Version - 10TH 07 edition

ISBN13: 978-0071254168

Alfred E. Brown

ISBN13: 978-0071254168
ISBN10: 0071254161
Cover type:
Edition/Copyright: 10TH 07
Publisher: McGraw-Hill Publishing Company

Published: 2007
International: No
Summary

The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and full-color format make Microbiological Applications: Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, this manual assumes no prior organic chemistry course has been taken.

Table of Contents

Part 1. Microscopy

Exercise 1. Brightfield Microscopy
Exercise 2. Darkfield Microscopy
Exercise 3. Phase-Contrast Microscopy
Exercise 4. Fluorescence Microscopy
Exercise 5. Microscopic Measurements

Part 2. Survey of Microorganisms

Exercise 6. Protozoans, Algae, and Cyanobacteria
Exercise 7. Ubiquity of Bacteria
Exercise 8. The Fungi: Yeasts and Molds

Part 3. Manipulation of Microorganisms

Exercise 9. Aseptic Technique
Exercise 10. Pure Culture Techniques

Part 4. Staining and Observation of Microorganisms

Exercise 11. Smear Preparation
Exercise 12. Simple Staining
Exercise 13. Negative Staining
Exercise 14. Capsular Staining
Exercise 15. Gram Staining
Exercise 16. Spore Staining: Two Methods
Exercise 17. Acid-Fast Staining: Ziehl-Neelsen Method
Exercise 18. Motility Determination

Part 5. Culture Methods

Exercise 19. Culture Media Preparation
Exercise 20. Preparation of Stock Cultures
Exercise 21. Cultivation of Anaerobes
Exercise 22. Enumeration of Bacteria: The Standard Plate Count
Exercise 23. Slime Mold Culture
Exercise 24. Slide Culture: Molds

Part 6. Bacterial Viruses

Exercise 25. Determination of a Bacteriophage Titer
Exercise 26. Burst Size Determination: A One-Step Growth Curve
Exercise 27. Isolation of Phage from Flies
Exercise 28. Phage Typing

Part 7. Enviornmental Influences and Control of Microbial Growth

Exercise 29. Temperature: Effects on Growth
Exercise 30. Temperature: Lethal Effects
Exercise 31. pH and Microbial Growth
Exercise 32. Water Activity and Osmotic Pressure
Exercise 33. Ultraviolet Light: Lethal Effects
Exercise 34. The Effects of Lysozyme on Bacterial Cells
Exercise 35. Evaluation of Alcohol: Its Effectiveness as an Antiseptic
Exercise 36. Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
Exercise 37. Evaluation of Antiseptics: The Filter Paper Disk Method
Exercise 38. Effectiveness of Hand Scrubbing

Part 8. Identification of Unknown Bacteria

Exercise 39. Morphological Study of Unknown Bacterium
Exercise 40. Cultural Characteristics
Exercise 41. Physiological Characteristics: Oxidation and Fermentation Tests
Exercise 42. Physiological Characteristics: Hydrolytic and Degradative Reactions
Exercise 43. Physiological Characteristics: Multiple Test Media
Exercise 44. Use of Bergey's Manual

Part 9. Miniaturized Multitest Systems

Exercise 45. Enterobacteriaceae Identification: The API 20E System
Exercise 46. Enterobacteriaceae Identification: The Enterotube II System
Exercise 47. O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System
Exercise 48. Staphyococcus Identification: The API Staph-Ident System

Part 10. Diversity and Environmental Microbiology

Exercise 49. Isolation of an Antibiotic Producer: The Actinomyces
Exercise 50. Nitrogen Cycle: Ammonification
Exercise 51. Symbiotic Nitrogen Fixation: Rhizobium
Exercise 52. Free-Living Nitrogen Fixation: Azotobacter
Exercise 53. Denitrification: Paracoccus Denitrificans
Exercise 54. The Winogradsky Column
Exercise 55. Purple Nonsulfer Photosynthetic Bacteria
Exercise 56. Sulfate Reducing Bacteria: Desulfovibrio
Exercise 57. Bacterial Commensalism
Exercise 58. Bacterial Synergism
Exercise 59. Microbial Antagonism

Part 11. Applied Microbiology

Exercise 60. Bacterial Counts of Foods
Exercise 61. Bacteriological Examination of Water: Qualitative Tests
Exercise 62. The Membrane Filter Method
Exercise 63. Reductase Test
Exercise 64. Microbial Spoilage of Canned Food
Exercise 65. Microbiology of Alcohol Fermentation

Part 12. Bacterial Genetics and Biotechnology

Exercise 66. Mutant Isolation by Replica Plating
Exercise 67. Bacterial Transformation
Exercise 68. Polymerase Chain Reaction for Amplifying DNA
Exercise 69. Plasmid Isolation

Part 13. Medical Microbiology and Immunology

Exercise 70. The Staphylococci: Isolation and Identification
Exercise 71. The Streptococci: Isolation and Identification
Exercise 72. Gram-Negative Intestinal Pathogens
Exercise 73. Slide Agglutination Test: Serological Typing
Exercise 74. Slide Agglutination (Latex) Test: For S. aureus Identification
Exercise 75. Tube Agglutination Test: The Heterophile Antibody Test
Exercise 76. White Blood Cell Study: The Differential WBC Count
Exercise 77. Blood Grouping
Exercise 78. A Synthetic Epidemic

Appendix A Tables

Appendix B Indicators, Stains, Reagents
Appendix C Media
Appendix D Identification Charts
Appendix E The Streptococci: Classification, Habitat, Pathology, and Biochemical Characteristics

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