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Experiments in Biochemistry : A Hands-on Approach - A Manual for the Undergraduate Laboratory

Experiments in Biochemistry : A Hands-on Approach - A Manual for the Undergraduate Laboratory - 00 edition

ISBN13: 978-0030212840

Cover of Experiments in Biochemistry : A Hands-on Approach - A Manual for the Undergraduate Laboratory 00 (ISBN 978-0030212840)
ISBN13: 978-0030212840
ISBN10: 0030212847
Cover type:
Edition/Copyright: 00
Publisher: Saunders College Division
Published: 2000
International: No

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Experiments in Biochemistry : A Hands-on Approach - A Manual for the Undergraduate Laboratory - 00 edition

ISBN13: 978-0030212840

Shawn O. Farrell

ISBN13: 978-0030212840
ISBN10: 0030212847
Cover type:
Edition/Copyright: 00
Publisher: Saunders College Division

Published: 2000
International: No
Summary

Alternate versions of experiments allow for easy in use in labs, which meet only once a week, or multiple times in a week.

  • Tip Boxes throughout text give students pointers on how to perform the labs.
  • Essential Information Boxes highlight pertinent information.
  • References and Further Reading sections are at the end of each chapter.
  • Webconnections site on Saunders web page provides links to useful biochemistry lab students.
  • Additional Problem Set in each chapter.

All experiments have been classroom tested through multiple semesters. All are proven to work and can be completed in a normal lab period.

Author Bio

Farrell, Shawn O. : Colorado State University

Table of Contents

Introduction to the Text
Objectives of the Biochemistry Laboratory
Chapter Format of Experiments in Biochemistry


Chapter 1 Biochemistry Boot Camp

Experiment #1 Use of Pipettors

Chapter 2 Acid, Bases, and Buffers

Experiment #2 Preparation of Buffers

Chapter 3 Spectrophotometry

Experiment #3 Beer's Law and Standard Curves
Experiment #3b Protein Concentration of LDH Fractions

Chapter 4 Enzyme Purification

Experiment #4 Purification of LDH (short version)
Experiment #4a Purification of LDH (comprehensive version)

Chapter 5 Ion Exchange Chromatography

Experiment #5 Separation and Identification of Amino Acids
Experiment #5a Purification of LDH with Ion Exchange Chromatography

Chapter 6 Affinity Chromatography

Experiment #6 Affinity Chromatography of LDH

Chapter 7 Gel Filtration Chromatography

Experiment #7 Gel Filtration Chromatography
Experiment #7a Gel Filtration Chromatography of LDH

Chapter 8 Enzyme Kinetics

Experiment #8 Enzyme Kinetics of Tyrosinase
Experiment #8a Enzyme Kinetics of LDH

Chapter 9 Electrophoresis

Experiment #9 Native Gel Separation of LDH Isozymes (short version)
Experiment #9a Native Gel Separation of LDH Isozymes (comprehensive)
Experiment #9b SDS-Polyacrylamide Gel Electrophoresis (short version)
Experiment #9c SDS-Polyacrylamide Gel Electrophoresis (comprehensive)

Chapter 10 Western Blots

Experiment #10 Western Blot of Serum Protein
Experiment #10a - Western Blot of LDH

Chapter 11 Restriction Enzymes

Experiment #11 Analysis of DNA Restriction Fragments

Chapter 12 Cloning and Expression of Foreign Proteins

Experiment #12 Cloning and Expression of Barracuda LDH-A

Chapter 13 Polymerase Chain Reaction

Experiment #13 PCR of Barracuda LDH-A

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Other Editions for Experiments in Biochemistry : A Hands-on Approach - A Manual for the Undergraduate Laboratory

Cover of Experiments in Biochemistry : Hands-on Approach 2nd edition

Experiments in Biochemistry : Hands-on Approach - 2nd edition

ISBN13: 978-0495013174
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