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Experiments in Biochemistry : Hands-on Approach

Experiments in Biochemistry : Hands-on Approach - 2nd edition

ISBN13: 978-0495013174

Cover of Experiments in Biochemistry : Hands-on Approach 2ND 06 (ISBN 978-0495013174)
ISBN13: 978-0495013174
ISBN10: 049501317X
Cover type: Paperback
Edition/Copyright: 2ND 06
Publisher: Brooks/Cole Publishing Co.
Published: 2006
International: No
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Experiments in Biochemistry : Hands-on Approach - 2ND 06 edition

ISBN13: 978-0495013174

Shawn O. Farrell and Lynn E. Taylor

ISBN13: 978-0495013174
ISBN10: 049501317X
Cover type: Paperback
Edition/Copyright: 2ND 06
Publisher: Brooks/Cole Publishing Co.

Published: 2006
International: No
Summary

EXPERIMENTS IN BIOCHEMISTRY: A HANDS-ON APPROACH, Second Edition features a variety of hands-on, classroom tested experiments that are proven to work and can be completed in a normal lab period. The manual's stand-alone experiments are effective in courses meeting only once a week, giving students a broad overview of the subject matter. A more comprehensive set of experiments is also available and allows students to delve further into each of the topics presented. The Second Edition also features new and revised experiments, including a new experiment that involves cloning the barracuda LDH gene! Students and professors will also find expanded problem sets in this edition. "Tip" boxes, located throughout the text, provide pointers to students on how to perform the experiment at hand, while "Essential Information" boxes highlight pertinent information that will help the student complete the experiment. The second edition continues to include references and further readings at the end of each chapter.

Benefits:

  • NEW! In Experiment 12, students will clone the barracuda LDH gene into an expression vector, making a HIS-Tagged fusion protein that can be purified by affinity chromatography. Students will create a new vector for doing expression that makes the ligation of DNA easier and more efficient while learning about expanded methods for purification of HIS-Tagged Lactate Dehydrogenase via Affinity Chromatography.
  • NEW! The Second Edition includes expanded problem sets for further biochemistry practice and study.
  • All experiments have been classroom tested through multiple semesters and can be completed in a normal lab period.
  • Alternate versions of experiments allow for easy use in labs which meet only once a week or multiple times in a week.
  • "Tip" boxes throughout text give students pointers on how to perform the labs.
  • "Essential Information" boxes highlight pertinent information.
  • "References and Further Reading" sections are at the end of each chapter.
  • An additional "Problem Set" appears in each chapter.

Table of Contents

Introduction to the Text.

Objectives of the Biochemistry Laboratory.

Chapter Format of Hands on Biochemistry.

1. Biochemistry Boot Camp.

2. Acids, Bases, and Buffers.

3. Spectrophotometry.

4. Enzyme Purification.

5. Ion Exchange Chromatography.

6. Affinity Chromatography.

7. Gel filtration Chromatography.

8. Enzyme Kinetics.

9. Electrophoresis.

10. Western Blots.

11. Restriction Enzymes.

12. Cloning and Expression of Foreign Proteins.

13. Polymerase Chain Reaction.

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