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Fundamental Laboratory Approaches for Biochemistry and Biotechnology

Fundamental Laboratory Approaches for Biochemistry and Biotechnology - 98 edition

ISBN13: 978-1891786006

Cover of Fundamental Laboratory Approaches for Biochemistry and Biotechnology 98 (ISBN 978-1891786006)
ISBN13: 978-1891786006
ISBN10: 1891786008
Cover type:
Edition/Copyright: 98
Publisher: Fitzgerald Science Press
Published: 1998
International: No

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Fundamental Laboratory Approaches for Biochemistry and Biotechnology - 98 edition

ISBN13: 978-1891786006

Alexander J. Ninfa and David P. Ballou

ISBN13: 978-1891786006
ISBN10: 1891786008
Cover type:
Edition/Copyright: 98
Publisher: Fitzgerald Science Press

Published: 1998
International: No
Summary

Fundamental Laboratory Approaches for Biochemistry and Biotechnology is fast becoming the laboratory text and combined manual of choice for students in biochemistry and other molecular life science courses. This exciting new book is being used at over 120 colleges and universities across the United States and Canada. Written by active scientists for advanced undergraduates and graduate students, the book provides readers with a logical framework for conducting modern biochemical and molecular biology research. The authors write with passion, and their love of biochemical research shines through on every page.

Features

  • 13 chapters on the underlying principles of biochemistry and molecular biology techniques.
  • 26 class-tested laboratory experiments covering basic methods.
  • Numerous examples of how ethical and scientific integrity issues might arise in research.
  • All mathematical topics include worked out sample problems and student exercises.
  • All reagents and supplies are listed with their Sigma Catalog numbers for ease of implementation.

Author Bio

Ninfa, Alexander J. : University of Michigan-Ann Arbor

Alexander J. Ninfa is Professor in the Department of Biological Chemistry at the University of Michigan. He won the University-wide award for excellence in teaching for the Biochemistry Laboratory course. He is known for his work on the mechanisms of signal transduction and transcriptional regulation in bacteria.

Ballou, David P. : University of Michigan-Ann Arbor

David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.

Table of Contents

CHAPTER 1: BASIC PRACTICES AND TECHNIQUES IN THE BIOCHEMISTRY LABORATORY

CHAPTER 2: SPECTROPHOTOMETRY

CHAPTER 3: QUANTIFICATION OF PROTEIN CONCENTRATION

CHAPTER 4: CHROMATOGRAPHY

CHAPTER 5: GEL ELECTROPHORESIS OF PROTEINS

CHAPTER 6: OVERVIEW OF PROTEIN PURIFICATION

CHAPTER 7: ISOLATION AND CHARACTERIZATION OF THE ENZYME ALKALINE PHOSPHATASE FROM ESCHERICHIA COLI

CHAPTER 8: ENZYME KINETICS

CHAPTER 9: ENZYMATIC METHODS OF ANALYSIS

CHAPTER 10: LIGAND BINDING

CHAPTER 11: RECOMBINANT DNA TECHNIQUES

CHAPTER 12: POLYMERASE CHAIN REACTION (PCR) TECHNOLOGY

CHAPTER 13: USING THE PERSONAL COMPUTER AND THE INTERNET FOR BIOCHEMICAL RESEARCH (RasMol, Chime, MedLine etc.)

APPENDIX A: SAFETY CHECKLIST

APPENDIX B: AN EXAMPLE OF AN MSDS

INDEX

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Cover of Fundamental Laboratory Approaches for Biochemistry and Biotechnology 2nd edition

Fundamental Laboratory Approaches for Biochemistry and Biotechnology - 2nd edition

ISBN13: 978-0470087664
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