Fundamental Laboratory Approaches For Biochemistry And Biotechnology - 98 edition

Ninfa, Alexander J. : University of Michigan-Ann Arbor

Alexander J. Ninfa is Professor in the Department of Biological Chemistry at the University of Michigan. He won the University-wide award for excellence in teaching for the Biochemistry Laboratory course. He is known for his work on the mechanisms of signal transduction and transcriptional regulation in bacteria.

Ballou, David P. : University of Michigan-Ann Arbor

David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.

"I have been looking for a text like this for four years. We have enthusiastically adopted it for our course."

--Linda Luck, Clarkson University

"I was very impressed with your book and think it fills a big gap in biochem lab texts! I have just given it to our bookstore to order for our fall biochem class. The molecular biology prof is also considering using it for our spring class!"

--Dr. Lisa Hedrick, University of St. Francis

"Besides the information about mechanisms and principles underlying various methods, the chapters on retrieving information from the Internet puts the book head and shoulders above other available books. I have adopted this book and have been recommending it to Biochemistry instructors all over the country."

--Probal Banerjee, College of Staten Island, City University of New York

"I'm quite impressed with the content. Not only are the experiments well written, the identification of the products at the end is a time saver that will be invaluable to any professor. I'm looking forward to adopting this text for our Biochemistry Lab course."

--Dr. Paul DeLaLuz, Lee University

Fitzgerald Science Press, Inc. Web Site, July, 2000

CHAPTER 1: BASIC PRACTICES AND TECHNIQUES IN THE BIOCHEMISTRY LABORATORY

CHAPTER 2: SPECTROPHOTOMETRY

CHAPTER 3: QUANTIFICATION OF PROTEIN CONCENTRATION

CHAPTER 4: CHROMATOGRAPHY

CHAPTER 5: GEL ELECTROPHORESIS OF PROTEINS

CHAPTER 6: OVERVIEW OF PROTEIN PURIFICATION

CHAPTER 7: ISOLATION AND CHARACTERIZATION OF THE ENZYME ALKALINE PHOSPHATASE FROM ESCHERICHIA COLI

CHAPTER 8: ENZYME KINETICS

CHAPTER 9: ENZYMATIC METHODS OF ANALYSIS

CHAPTER 10: LIGAND BINDING

CHAPTER 11: RECOMBINANT DNA TECHNIQUES

CHAPTER 12: POLYMERASE CHAIN REACTION (PCR) TECHNOLOGY

CHAPTER 13: USING THE PERSONAL COMPUTER AND THE INTERNET FOR BIOCHEMICAL RESEARCH (RasMol, Chime, MedLine etc.)

APPENDIX A: SAFETY CHECKLIST

APPENDIX B: AN EXAMPLE OF AN MSDS

INDEX