Genomes - 2nd edition

An Introduction to Genomes.

PART 1: Genomes,Transcriptomes and Proteomes.

Chapter 1 The Human Genome.

1.1 DNA.
1.2 The Human Genome.
1.3 Why is The Human Genome Project Important?

Chapter 2 Genome Anatomies.

2.1 An Overview of Genome Anatomies.
2.3 The Anatomy of The Prokaryotic Genome.
2.4 The Repetitive DNA Content of Genomes.

Chapter 3 Transcriptomes and Proteomes.

3.1 Genome Expression in Outline.
3.3 The Protein Content of the Cell.

PART 2: Studying Genomes.

Chapter 4 Studying DNA.

4.1 Enzymes for DNA Manipulation.
4.2 DNA Cloning.
4.3 The Polymerase Chain Reaction (PCR).

Chapter 5 Mapping Genomes.

5.1 Genetic and Physical Maps.
5.2 Genetic Mapping.
5.3 Physical Mapping

Chapter 6 Sequencing Genomes.

6.1 The Methodology for DNA Sequencing.
6.2 Assembly of a Contiguous DNA Sequence.
6.3 The Human Genome Projects.

Chapter 7 Understanding a Genome Sequence.

7.1 Locating the Genes in a Genome Sequence.
7.2 Determining the Functions of Individual Genes.
7.3 Global Studies of Genome Activity.
7.4 Comparative Genomics.

PART 3: How Genomes Function.

Chapter 8 Accessing the Genome.

8.1 Inside the Nucleus.
8.2 Chromatin Modifications and Genome Expression.

Chapter 9 Assembly of the Transcription Initiation Complex.

9.1 The Importance of DNA-binding Proteins.
9.2 DNAprotein Interactions During Transcription Initiation.
9.3 Regulation of Transcription Initiation.

Chapter 10 Synthesis and Processing of RNA.

10.1 Synthesis and processing of mRNA.
10.2 Synthesis and processing of non-coding RNAs.
10.3 Processing of Pre-RNA by Chemical Modification.
10.4 Degradation of mRNAs.
10.5 Transport of RNA Within the Eukaryotic Cell.

Chapter 11 Synthesis and Processing of the Proteome.

11.1 The Role of tRNA in Protein Synthesis.
11.2 The Role of the Ribosome in Protein Synthesis.
11.3 Post-translational Processing of Proteins.
11.4 Protein Degradation.

Chapter 12 Regulation of Genome Activity.

12.1 Transient Changes in Genome Activity.
12.2 Permanent and Semi-Permanent Changes in Genome Activity.
12.3 Regulation of Genome Activity During Development.

PART 4 How Genomes Replicate and Evolve.

Chapter 13 Genome Replication.

13.1 The Topologic Problem.

Chapter 14 Mutation, Repair and Recombination.

14.1 Mutations.
14.2 DNA Repair.
14.3 Recombination.

Chapter 15 How Genomes Evolve.

15.1 Genomes: the First Ten Billion Years.
15.2 Acquisition of New Genes.
15.3 Non-coding DNA and Genome Evolution.
15.4 The Human Genome: the Last Five Million Years.

Chapter 16 Molecular Phylogenetics.

16.1 The Origins of Molecular Phylogenetics.
16.2 The Reconstruction of DNA-based Phylogenetic Trees.

Appendix: Keeping up to Date.
Glossary.
Index.