Summary: This vivid, full-color handbook guides students in manipulations and preparations needed in the microbiology laboratory. The approach is visual and incorporates ''voice balloons'' that keep the student focused on the process described. The techniques are those that will be used frequently for studying microbes in the laboratory, and include those identified by the American Society for Microbiology in its recommendations for the Microbiology Laboratory Core Curriculu
Summary: This vivid, full-color handbook guides students in manipulations and preparations needed in the microbiology laboratory. The approach is visual and incorporates ''voice balloons'' that keep the student focused on the process described. The techniques are those that will be used frequently for studying microbes in the laboratory, and include those identified by the American Society for Microbiology in its recommendations for the Microbiology Laboratory Core Curriculum. ...show less
Edition/Copyright:07 Cover: Spiral Publisher:Prentice Hall, Inc. Published: 10/29/2006 International: No
View Table of Contents
Unit I. Safety first!
Safety in the microbiology laboratory Universal precautions
Unit II. Culturing bacteria and aseptic techniques
Preparing culture media Sterilization The autoclave Dry heat Aseptic transfer of bacteria Adjusting the gas burner Preparing bacterial cultures in broth tubes and on agar slants Transfer colony from plate to broth and to agar slant Isolation of bacteria Preparing a pour plate Preparing a streak plate Preparing a spread plate Characteristic features of bacterial growth in broth and on agar Maintenance and storage of stock cultures Culturing anaerobic bacteria
Unit III. Visualizing bacteria
Effective use and responsible care of the light microscope Measuring microscopic cells Preparing a hanging-drop slide Preparing a bacterial smear Preparing a simple stain Preparing a Gram stain Preparing an acid-fast stain Preparing a negative stain Preparing a capsule stain Preparing an endospore stain
Unit IV. Enzyme-based tests for the identification of bacteria
Hydrolytic (digestive) enzymes Starch hydrolysis Casein hydrolysis Gelatin hydrolysis Lipid hydrolysis (tributyrin and spirit blue agar) DNA hydrolysis Utilization of carbohydrates Fermentation of carbohydrates (Durham tube) Methyl red test (mixed fermentation) Voges-Proskauer test (butanediol fermentation) Citrate utilization Oxidation-fermentation (O-F) glucose test Degradation of amino acids Indole test H2S production Phenylalanine deamination Decarboxylase test Respiration tests Catalase test Oxidase test Nitrate reduction Miscellaneous tests Triple sugar iron (TSI) test Urea hydrolysis Litmus milk reactions Motility assay Coagulase production Selective and/or differential media Blood agar Eosin methylene blue (EMB) agar Mannitol-salt agar MacConkey agar Phenylethyl alcohol (PEA) agar
Unit V. Counting microbes
Direct microscopic counting with the Petroff-Hausser chamber Preparing a standard plate count of bacteria Using Turbidemetry to estimate cell density Plaque assay for determining bacteriophage titer
Unit VI. Measuring effectiveness of antibacterial chemicals
Kirby-Bauer method for sensitivity of bacteria to antibacterial medicines Evaluating antibacterial chemicals: The disk-diffusion method
Barnes & Noble.com Various Shipping Locations, USA
Brand new, ships from the closest shipping location to your shipping address!
$17.91 +$3.99 s/h
Goodwill of Orange County Santa Ana, CA
Acceptable Book has internal/external wear and/or highlighting and underlining.
$23.75 +$3.99 s/h
Textbookcenter.com Columbia, MO
Ships same day or next business day via UPS (Priority Mail for AK/HI/APO/PO Boxes)! Used sticker and some writing and/or highlighting. Used books may not include working access code or dust jacket.
$23.94 +$3.99 s/h
BookCellar-NH Nashua, NH
0132240114 Has moderate shelf and/or corner wear. Great used condition. We are a tested and proven company with over 900,000 satisfied customers since 1997. We ship daily M-F. Choose expedited shippin...show moreg (if available) for much faster delivery. Delivery confirmation on all US orders. ...show less
$25.87 +$3.99 s/h
PhatCampus Miami Lakes, FL
Used Condition and may contain writing/highlighting. May NOT contain CD/ DVD/ Access Code/ Workbook etc
$27.60 +$3.99 s/h
HPB-McKinney McKinney, TX
2006 Paperback Good Connecting readers with great books since 1972. Used books may not include companion materials, some shelf wear, may contain highlighting/notes, and may not include cd-rom or ac...show morecess codes. Customer service is our top priority! ...show less
$28.79 +$3.99 s/h
PaperbackshopUS Secaucus, NJ
Used - Very Good Book. Shipped from US within 4 to 14 business days. Established seller since 2000
$32.83 +$3.99 s/h
Textbook Charlie Nashville, TN
Brand New! Ships same day or next business day. Free USPS Tracking Number. Excellent Customer Service. Ships from TN
$34.42 +$3.99 s/h
GreatBookPrices Westminster, MD
Used, Acceptable Condition, may show signs of wear and previous use. Please allow 4-14 business days for delivery. 100% Money Back Guarantee, Over 1,000,000 customers served.
$38.01 +$3.99 s/h
PaperbackshopUS Secaucus, NJ
New Book. Shipped from UK within 10 to 14 business days. Established seller since 2000.
Free Shipping Get Free Shipping on orders over $25 (not including Rental and Marketplace). Order arrives in 5-10 business days.
Need it faster? We offer fast, flat-rate expedited shipping options.
Not the right book for you? We'll gladly take it back within 30 days.
To return an eTextbook:
Your eTextbook is non-returnable once it's been activated. You must contact us about returning your eTextbook before you activate it.
Returns are accepted within 30 days of the purchase date on your order confirmation.
This book qualifies for guaranteed cash back! Buy it now for , then:
Sell it back by:
Guaranteed cash back:
Cost of this book after cash back:
Take advantage of Guaranteed Cash Back. Send your book to us in good condition before the end of the buyback period, we'll send YOU a check, and you'll pay less for your textbooks!
If you find this book for less on Amazon.com (direct from Amazon, not marketplace sellers), we'll match it.
In our warehouse, waiting to ship directly to you.
We hand-inspect every used textbook to make sure it's in good condition.
Buy it now. Sell it later!
Sell this textbook for cash!
When you're done with this book, sell it back to Textbooks.com. In addition to the best possible buyback price, you'll get an extra 10% cash back just for being a customer.
We buy good-condition used textbooks year 'round, 24/7. No matter where you bought it, Textbooks.com will buy your textbooks for the most cash.
We hand-inspect every one of our used textbooks to ensure good condition.
Our used textbooks do NOT have:
Missing or torn pages
Missing or torn cover
Torn or damaged binding
A broken spine
This textbook has never been used.
Due to the size of eTextbooks, a high-speed internet connection (cable modem, DSL, LAN) is required for download stability and speed. Your connection can be wired or wireless.
Being online is not required for reading an eTextbook after successfully downloading it. You must only be connected to the Internet duringthe download process.
XP or Windows 7 (32 or 64 running in 32 bit mode), or Mac OS 10.6 or above
At least 512 MB RAM, 600 mHZ processor, and 40 MB of hard drive space (75MB for Mac OS)
What is the Marketplace? It's another way for you to get the right price on the books you need. We approved every Marketplace vendor to sell their books on Textbooks.com, so you know they're all reliable.
What are Marketplace shipping options? Marketplace items do not qualify for free shipping. When ordering from the Marketplace, please specify whether you want the seller to send your book Standard ($3.99/item) or Express ($6.99/item). To get free shipping over $25, just order directly from Textbooks.com instead of through the Marketplace.
FREE UPS 2nd Day Air Terms
Rental and Marketplace items are excluded. Offer is valid from 1/21/2013 12:00PM to 1/23/2013 11:59AM CST. Your order must be placed by 12 Noon CST to be processed on the same day. Minimum order value is $100.00 excluding Rental and Marketplace items. To redeem this offer, select "FREE UPS 2ND DAY AIR" at checkout. Offer not is not valid on previous orders.