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Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Short Version

Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Short Version - 10th edition

Benson's Microbiological Applications : Laboratory Manual in General Microbiology, Short Version - 10th edition

ISBN13: 9780072992724

ISBN10: 0072992727

Edition: 10TH 07
Copyright: 2007
Publisher: McGraw-Hill Publishing Company
International: No

ISBN13: 9780072992724

ISBN10: 0072992727

Edition: 10TH 07

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The classic resource for undergraduate microbiology laboratory courses just keeps getting better. The self-contained, clearly illustrated exercises and four-color format make Microbiological Applications: Laboratory Manual in General Microbiology the ideal lab manual. Appropriate for either a majors or non-majors lab course, this lab manual assumes no prior organic chemistry course has been taken.

New Features

  • Content has been updated throughout, especially in Part 8: Identification of Unknown Bacteria, and in Part 11: Medical Microbiology and Immunology, to include the latest research. Also updated was Exercise 60: A Synthetic Epidemic, in keeping with lab safety requirements.
  • One of the major changes to the 10/e is that the lab report sections have been extensively revised - all of the lab reports have been updated with new questions.
  • The introductory section for phase-contrast microscopy has been revised with a new discussion of the optics and workings of the phase contrast microscope. Exercise 3
  • The exercises on evaluation of antibiotics, antiseptics, and disinfectants have been revised to include definitions of antimicrobials, antibiotics, antiseptics, and disinfectants. Exercises 33 and 34
  • A new exercise on lysozyme employs egg white as a source of this enzyme to demonstrate that lysozyme breaks down peptidoglycan in bacterial cells, making the bacteria prone to lysis. This exercise will demonstrate the difference in sensitivity of Gram-positive and Gram-negative bacteria to lysozyme as a result of the presence of the outer membrane that surrounds Gram-negative cells. Exercise 31
  • The exercises on the identification of an unknown bacterium have been rewritten and reorganized into exercises that separate the tests into categories based on fermentation, oxidation, degradative and hydrolytic enzymes, and multiple test media. The O/F glucose test has been added to the fermentation tests and a complete description the various reactions for Kleiger's iron agar is included. 38, 39, 40
  • The classification of bacteria in the tenth edition is consistent with that found in the first edition of Bergey's Manual of Systematic Bacteriology. 52, 53, and 54

Table of Contents

Table of Contents

Part 1 Microscopy

Exercise 1 Brightfield Microscopy
Exercise 2 Darkfield Microscopy
Exercise 3 Phase-Contrast Microscopy
Exercise 4 Microscopic Measurements

Part 2 Survey of Microorganisms

Exercise 5 Protozoans, Algae, and Cyanobacteria
Exercise 6 Ubiquity of Bacteria
Exercise 7 The Fungi: Yeasts and Molds

Part 3 Manipulation of Microorganisms

Exercise 8 Aseptic Technique
Exercise 9 Pure Culture Techniques

Part 4 Staining and Observation of Microorganisms

Exercise 10 Smear Preparation
Exercise 11 Simple Staining
Exercise 12 Negative Staining
Exercise 13 Capsular Staining
Exercise 14 Gram Staining
Exercise 15 Spore Staining: Two Methods
Exercise 16 Acid-Fast Staining: Ziehl-Neelsen Method
Exercise 17 Motility Determination

Part 5 Culture Methods

Exercise 18 Culture Media Preparation
Exercise 19 Cultivation of Anaerobes
Exercise 20 Enumeration of Bacteria: The Standard Plate Count
Exercise 21 Slime Mold Culture
Exercise 22 Slide Culture: Molds

Part 6 Bacterial Viruses

Exercise 23 Determination of a Bacteriophage Titer
Exercise 24 Isolation of Phage from Flies
Exercise 25 Phage Typing

Part 7 Enviornmental Influences and Control of Microbial Growth

Exercise 26 Temperature: Effects on Growth
Exercise 27 Temperature: Lethal Effects
Exercise 28 pH and Microbial Growth
Exercise 29 Water Activity and Osmotic Pressure
Exercise 30 Ultraviolet Light: Lethal Effects
Exercise 31 Oligodynamic Action
Exercise 32 Evaluation of Alcohol: Its Effectiveness As an Antiseptic
Exercise 33 Antimicrobic Sensitivity Testing: The Kirby-Bauer Method
Exercise 34 Evaluation of Antiseptics: The Filter Paper Disk Method
Exercise 35 Effectiveness of Hand Scrubbing

Part 8 Identification of Unknown Bacteria

Exercise 36 Morphological Study of Unknown Bacterium
Exercise 37 Cultural Characteristics
Exercise 38 Physiological Characteristics: Oxidation and Fermentation Tests
Exercise 39 Physiological Characteristics: Hydrolytic and Degradative Reactions
Exercise 40 Physiological Characteristics: Multiple Test Media
Exercise 41 Use of Bergey's Manual

Part 9 Miniaturized Multitest Systems

Exercise 42 Enterobacteriaceae Identification: The API 20E System
Exercise 43 Enterobacteriaceae Identification: The Enterotube II System
Exercise 44 O/F Gram-Negative Rods Identification: The Oxi/Ferm Tube II System
Exercise 45 Staphyococcus Identification: The API Staph-Ident System

Part 10 Diversity and Environmental Microbiology

Exercise 46 Bacterial Counts of Foods
Exercise 47 Bacteriological Examination of Water: Qualitative Tests
Exercise 48 The Membrane Filter Method
Exercise 49 Reductase Test
Exercise 50 Microbial Spoilage of Canned Food
Exercise 51 Microbiology of Alcohol Fermentation

Part 11 Medical Microbiology and Immunology

Exercise 52 The Staphylococci: Isolation and Identification
Exercise 53 The Streptococci: Isolation and Identification
Exercise 54 Gram-Negative Intestinal Pathogens
Exercise 55 Slide Agglutination Test: Serological Typing
Exercise 56 Slide Agglutination (Latex) Test: For S. aureus Identification
Exercise 57 Tube Agglutination Test: The Heterophile Antibody Test
Exercise 58 White Blood Cell Study: The Differential WBC Count
Exercise 59 Blood Grouping
Exercise 60 A Synthetic Epidemic