Fundamental Laboratory Approaches for Biochemistry and Biotechnology - 98 edition
Fundamental Laboratory Approaches for Biochemistry and Biotechnology - 98 edition
ISBN13: 9781891786006
ISBN10: 1891786008
by Alexander J. Ninfa and David P. Ballou
Edition: 98Copyright: 1998
Publisher: Fitzgerald Science Press
Published: 1998
International: No
This title is currently not available in digital format.
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Summary
Fundamental Laboratory Approaches for Biochemistry and Biotechnology is fast becoming the laboratory text and combined manual of choice for students in biochemistry and other molecular life science courses. This exciting new book is being used at over 120 colleges and universities across the United States and Canada. Written by active scientists for advanced undergraduates and graduate students, the book provides readers with a logical framework for conducting modern biochemical and molecular biology research. The authors write with passion, and their love of biochemical research shines through on every page.
Features
- 13 chapters on the underlying principles of biochemistry and molecular biology techniques.
- 26 class-tested laboratory experiments covering basic methods.
- Numerous examples of how ethical and scientific integrity issues might arise in research.
- All mathematical topics include worked out sample problems and student exercises.
- All reagents and supplies are listed with their Sigma Catalog numbers for ease of implementation.
Author Bio
Ninfa, Alexander J. : University of Michigan-Ann Arbor
Alexander J. Ninfa is Professor in the Department of Biological Chemistry at the University of Michigan. He won the University-wide award for excellence in teaching for the Biochemistry Laboratory course. He is known for his work on the mechanisms of signal transduction and transcriptional regulation in bacteria.
Ballou, David P. : University of Michigan-Ann Arbor
David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.
Table of Contents
CHAPTER 1: BASIC PRACTICES AND TECHNIQUES IN THE BIOCHEMISTRY LABORATORY
CHAPTER 2: SPECTROPHOTOMETRY
CHAPTER 3: QUANTIFICATION OF PROTEIN CONCENTRATION
CHAPTER 4: CHROMATOGRAPHY
CHAPTER 5: GEL ELECTROPHORESIS OF PROTEINS
CHAPTER 6: OVERVIEW OF PROTEIN PURIFICATION
CHAPTER 7: ISOLATION AND CHARACTERIZATION OF THE ENZYME ALKALINE PHOSPHATASE FROM ESCHERICHIA COLI
CHAPTER 8: ENZYME KINETICS
CHAPTER 9: ENZYMATIC METHODS OF ANALYSIS
CHAPTER 10: LIGAND BINDING
CHAPTER 11: RECOMBINANT DNA TECHNIQUES
CHAPTER 12: POLYMERASE CHAIN REACTION (PCR) TECHNOLOGY
CHAPTER 13: USING THE PERSONAL COMPUTER AND THE INTERNET FOR BIOCHEMICAL RESEARCH (RasMol, Chime, MedLine etc.)
APPENDIX A: SAFETY CHECKLIST
APPENDIX B: AN EXAMPLE OF AN MSDS
INDEX
Other Editions of Fundamental Laboratory Approaches for Biochemistry and Biotechnology
shop us with confidence
Fundamental Laboratory Approaches for Biochemistry and Biotechnology is fast becoming the laboratory text and combined manual of choice for students in biochemistry and other molecular life science courses. This exciting new book is being used at over 120 colleges and universities across the United States and Canada. Written by active scientists for advanced undergraduates and graduate students, the book provides readers with a logical framework for conducting modern biochemical and molecular biology research. The authors write with passion, and their love of biochemical research shines through on every page.
Features
- 13 chapters on the underlying principles of biochemistry and molecular biology techniques.
- 26 class-tested laboratory experiments covering basic methods.
- Numerous examples of how ethical and scientific integrity issues might arise in research.
- All mathematical topics include worked out sample problems and student exercises.
- All reagents and supplies are listed with their Sigma Catalog numbers for ease of implementation.
Ninfa, Alexander J. : University of Michigan-Ann Arbor
Alexander J. Ninfa is Professor in the Department of Biological Chemistry at the University of Michigan. He won the University-wide award for excellence in teaching for the Biochemistry Laboratory course. He is known for his work on the mechanisms of signal transduction and transcriptional regulation in bacteria.
Ballou, David P. : University of Michigan-Ann Arbor
David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.
CHAPTER 1: BASIC PRACTICES AND TECHNIQUES IN THE BIOCHEMISTRY LABORATORY
CHAPTER 2: SPECTROPHOTOMETRY
CHAPTER 3: QUANTIFICATION OF PROTEIN CONCENTRATION
CHAPTER 4: CHROMATOGRAPHY
CHAPTER 5: GEL ELECTROPHORESIS OF PROTEINS
CHAPTER 6: OVERVIEW OF PROTEIN PURIFICATION
CHAPTER 7: ISOLATION AND CHARACTERIZATION OF THE ENZYME ALKALINE PHOSPHATASE FROM ESCHERICHIA COLI
CHAPTER 8: ENZYME KINETICS
CHAPTER 9: ENZYMATIC METHODS OF ANALYSIS
CHAPTER 10: LIGAND BINDING
CHAPTER 11: RECOMBINANT DNA TECHNIQUES
CHAPTER 12: POLYMERASE CHAIN REACTION (PCR) TECHNOLOGY
CHAPTER 13: USING THE PERSONAL COMPUTER AND THE INTERNET FOR BIOCHEMICAL RESEARCH (RasMol, Chime, MedLine etc.)
APPENDIX A: SAFETY CHECKLIST
APPENDIX B: AN EXAMPLE OF AN MSDS
INDEX
Published: 1998
International: No
Summary
Fundamental Laboratory Approaches for Biochemistry and Biotechnology is fast becoming the laboratory text and combined manual of choice for students in biochemistry and other molecular life science courses. This exciting new book is being used at over 120 colleges and universities across the United States and Canada. Written by active scientists for advanced undergraduates and graduate students, the book provides readers with a logical framework for conducting modern biochemical and molecular biology research. The authors write with passion, and their love of biochemical research shines through on every page.
Features
- 13 chapters on the underlying principles of biochemistry and molecular biology techniques.
- 26 class-tested laboratory experiments covering basic methods.
- Numerous examples of how ethical and scientific integrity issues might arise in research.
- All mathematical topics include worked out sample problems and student exercises.
- All reagents and supplies are listed with their Sigma Catalog numbers for ease of implementation.
Author Bio
Ninfa, Alexander J. : University of Michigan-Ann Arbor
Alexander J. Ninfa is Professor in the Department of Biological Chemistry at the University of Michigan. He won the University-wide award for excellence in teaching for the Biochemistry Laboratory course. He is known for his work on the mechanisms of signal transduction and transcriptional regulation in bacteria.
Ballou, David P. : University of Michigan-Ann Arbor
David P. Ballou is Professor in the Department of Biological Chemistry at the University of Michigan and is widely known for his work in rapid kinetics and the study of enzyme mechanisms involving redox coenzymes.
Table of Contents
Table of Contents
CHAPTER 1: BASIC PRACTICES AND TECHNIQUES IN THE BIOCHEMISTRY LABORATORY
CHAPTER 2: SPECTROPHOTOMETRY
CHAPTER 3: QUANTIFICATION OF PROTEIN CONCENTRATION
CHAPTER 4: CHROMATOGRAPHY
CHAPTER 5: GEL ELECTROPHORESIS OF PROTEINS
CHAPTER 6: OVERVIEW OF PROTEIN PURIFICATION
CHAPTER 7: ISOLATION AND CHARACTERIZATION OF THE ENZYME ALKALINE PHOSPHATASE FROM ESCHERICHIA COLI
CHAPTER 8: ENZYME KINETICS
CHAPTER 9: ENZYMATIC METHODS OF ANALYSIS
CHAPTER 10: LIGAND BINDING
CHAPTER 11: RECOMBINANT DNA TECHNIQUES
CHAPTER 12: POLYMERASE CHAIN REACTION (PCR) TECHNOLOGY
CHAPTER 13: USING THE PERSONAL COMPUTER AND THE INTERNET FOR BIOCHEMICAL RESEARCH (RasMol, Chime, MedLine etc.)
APPENDIX A: SAFETY CHECKLIST
APPENDIX B: AN EXAMPLE OF AN MSDS
INDEX
