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Microbiology : Laboratory Theory and Application

Microbiology : Laboratory Theory and Application - 2nd edition

Microbiology : Laboratory Theory and Application - 2nd edition

ISBN13: 9780895827081

ISBN10: 0895827085

Microbiology : Laboratory Theory and Application by Michael Leboffe and Burton Pierce - ISBN 9780895827081
Edition: 2ND 06
Copyright: 2006
Publisher: Morton Publishing Co.
International: No
Microbiology : Laboratory Theory and Application by Michael Leboffe and Burton Pierce - ISBN 9780895827081

ISBN13: 9780895827081

ISBN10: 0895827085

Edition: 2ND 06

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This full-color laboratory manual is designed for major and non-major students taking an introductory level microbiology lab course. Whether your course caters to pre-health professional students, microbiology majors or pre-med students, everything they need for a thorough introduction to the subject of microbiology is right here.

The new edition features many new or enhanced photographs throughout the book. Many exercises have been updated to increase understandability, to produce better results, and to increase student success. A detailed list of second edition changes can be found in the Preface on page iii.

The manual emphasizes content and skills as advised by the American Society for Microbiology in their Laboratory Core Curriculum. The lab experiments, which have been chosen to provide exposure to lab experiences from all areas of microbiology, will allow your students to develop insight into the process of science and experience some of the excitement associated with using a scientific approach to answering questions.

Table of Contents

Table of Contents

1. Fundamental Skills for the Microbiology Laboratory

Basic Growth Media
Exercise 1-1 Nutrent Broth and Nutrient Agar Preparation
Common Aseptic Transfers and Inoculation Methods
Exercise 1-2 Common Aseptic Transfers and Inoculation Methods
Exercise 1-3 Streak Plate Methods of Isolation
Exercise 1-4 Spread Plate Method of Isolation

2. Microbial Growth

Diversity and Ubiquity of Microorganisms
Exercise 2-1 Ubiquity of Microorganisms
Exercise 2-2 Colony Morphology
Exercise 2-3 Growth Patterns on Slants
Exercise 2-4 Growth Patterns in Broth
Environmental Factors Affecting Microbial Growth
Exercise 2-5 Evaluation of Media
Exercise 2-6 Agar Deep Stabs
Exercise 2-7 Agar Shakes
Exercise 2-8 Fluid Thioglycollate Medium
Exercise 2-9 Anaerobic Jar
Exercise 2-10 The Effect of Temperature on Microbial Growth
Exercise 2-11 The Effect of pH on Bacterial Growth
Exercise 2-12 The Effect of Osmotic Pressure on Microbial Growth
Control of Microorganisms
Exercise 2-13 The Lethal Effect of Ultraviolet Light on Microbial Growth
Exercise 2-14 Use Dilution: Measuring Disinfectant Effectivness
Exercise 2-15 Effectiveness of Hand Scrubbing

3. Microscopy and Staining

Exercise 3-1 Introduction to the Light Microscope
Exercise 3-2 Calibration of the Ocular Micrometer
Exercise 3-3 Examination of Eukaryotic Microbes
Bacterial Structure and Simple Stains
Exercise 3-4 Simple Stains
Exercise 3-5 Negative Stain
Differential Stains
Exercise 3-6 Gram Stain
Exercise 3-7 Acid-Fast Stains
Exercise 3-8 Capsule Stain
Exercise 3-9 Endospore Stain
Exercise 3-10 Wet Mount and Hanging Drop Preparations
Exercise 3-11 Flagella Stain
Exercise 3-12 Morphological Unknown

4. Selective Media

Selective Media for Isolation of Gram-Positive Cocci
Exercise 4-1 Mannitol Salt Agar
Exercise 4-2 Phenylethyl Alcohol Agar
Selective Media for Isolation of Gram-Negative Rods
Exercise 4-3 Desoxycholate Agar
Exercise 4-4 Endo Agar
Exercise 4-5 Eosin Methylene Blue Agar
Exercise 4-6 Hektoen Enteric Agar
Exercise 4-7 MacConkey Agar
Exercise 4-8 Xylose Lysine Desoxycholate Agar

5. Differential Tests

Introduction to Energy Metabolism Tests
Exercise 5-1 Oxidation-Fermentation Test
Fermentation Tests
Exercise 5-2 Phenol Red Broth
Exercise 5-3 Purple Broth
Exercise 5-4 Methyl Red and Voges-Proskauer Tests
Tests Identifying Microbial Ability to Respire
Exercise 5-5 Catalase Test
Exercise 5-6 Oxidase Test
Exercise 5-7 Nitrate Reduction Test
Utilization Media
Exercise 5-8 Citrate Test
Exercise 5-9 Malonate Test
Decarboxylation and Deamination Tests
Exercise 5-10 Decarboxylation Test
Exercise 5-11 Phenylalanine Deaminase Test
Tests Detecting the Presence of Hydrolytic Enzymes
Exercise 5-12 Bile Esculin Test
Exercise 5-13 Starch Hydrolysis
Exercise 5-14 ONPG Test
Exercise 5-15 Urease Tests
Exercise 5-16 Casease Test
Exercise 5-17 Gelatinase Test
Exercise 5-18 DNase Test
Exercise 5-19 Lipase Test
Combination Differential Media
Exercise 5-20 SIM Medium
Exercise 5-21 Triple Sugar Iron Agar
Exercise 5-22 Lysine Iron Agar
Exercise 5-23 Litmus Milk Medium
Antimicrobial Susceptibility and Resistance
Exercise 5-24 Bacitracin Susceptibility Test
Exercise 5-25 ß-Lactamase Test
Other Differential Tests
Exercise 5-26 Blood Agar
Exercise 5-27 Coagulase Tests
Exercise 5-28 Motility Test
Multiple Test Systems
Exercise 5-29 API 20 E Identification System for Enterobacteriaceae and Other Gram-Negative Rods
Exercise 5-30 Enterotube® II
Bacterial Unknowns Project
Exercise 5-31 Bacterial Unknowns Project

6. Quantitative Techniques

Exercise 6-1 Standard Plate Count (Viable Count)
Exercise 6-2 Urine Culture
Exercise 6-3 Direct Count (Petroff-Hausser)
Exercise 6-4 Plaque Assay
Exercise 6-5 Closed-System Growth
Exercise 6-6 Thermal Death Time Versus Decimal Reduction Value

7. Medical Microbiology

Exercise 7-1 Snyder Test
Exercise 7-2 Lysozyme Assay
Exercise 7-3 Antimicrobial Susceptibility Test
Exercise 7-4 MMWR Report
Exercise 7-5 Epidemic Simulation
Identification Flowcharts
Exercise 7-6 Identification of Enterobacteriaceae
Exercise 7-7 Identification of Gram-positive Cocci

8. Food and Environmental Biology

Environmental Microbiology
Exercise 8-1 Membrane Filter Technique
Exercise 8-2 Multiple Tube Fermentation Method for Total Coliform Determination
Exercise 8-3 Bioluminesence
Exercise 8-4 Soil Microbial Count
Food Microbiology
Exercise 8-5 Methylene Blue Reductase Test
Exercise 8-6 Viable Cell Preservatives
Exercise 8-7 Making Yogurt

9. Microbial Genetics

Exercise 9-1 Extraction of DNA from Bacterial Cells
Exercise 9-2 Ultraviolet Radiation Damage and Repair
Exercise 9-3 Ames Test
Exercise 9-4 Bacterial Transformation The pGLO System

10. Hematology and Serology

Exercise 10-1 Differential Blood Cell Count
Simple Serological Reactions
Exercise 10-2 Precipitin Ring Test
Exercise 10-3 Gel Immunodiffusion
Exercise 10-4 Slide Agglutination
Exercise 10-5 Blood Typing

11. Eukaryotic Microbes

Exercise 11-1 The Fungi: Common Yeasts and Molds
Exercise 11-2 Examination of Common Protozoans of Clinical Importance
Exercise 11-3 Parasitic Helminths
Appendix A: Biochemical Pathways
Appendix B: Miscellaneous Transfer Methods
Appendix C: Using Glass Pipettes to Transfer from a Broth Culture
Appendix D: Transfers from a Broth Culture Using a Digital Pipette
Appendix E: The Spectrophotometer
Appendix F: Alternative Procedures for Section 6
Appendix G: Media, Reagent, and Stain Recipes
Data Sheets